Sodium azide enhancement of interleukin-2 production.
نویسندگان
چکیده
The production of large quantities of Interleukin-2 (IL-2) from normal human lymphocytes has been limited by the short production and release period, as well as its absorption by the responsive cell population. We report the utilization of sodium azide (NaN3, 0.01% final concentration) to allow continued production of IL-2 as long as 72-96 hr and thereby increase the yield significantly. Cell cycle analysis performed by flow cytometry indicates that NaN3 blocks cells at the G0-G1 transition and the G1-S transition, depending on the time of addition of NaN3 to the cultures.
منابع مشابه
Mutagenic interactions in combined treatments of ethylmethane sulphonate,diethyl sulohate and sodium azide on barley
متن کامل
Reaction of Sodium Azide with 1,1'–Dicyclohexenyl–Diepoxides
Nucleophilic ring opening of dl and meso 1,1¢- dicyclohexenyl diepoxides by sodium azide in dioxane and aqueous alcoholic medium give the corresponding azidoalcohols. The obtention of departure diepoxides from diazidoalcohols by the reaction with sodium hydride shows the anti position of OH and N3 groups.
متن کاملمروری بر روشهای سنتز آلکیلآزیدها و کاربردهای آنها در صنایع نظامی
Azide compounds have vast applications in military and civilian industries. Some of their applications include use in drug or drug intermediate production, macro cyclic polymer production through click reaction, automobile airbag production, energetic binders such as GAP, oxidizer, plasticizer and burning rate control additive in solid composite propellants. Due to the wide range of appli...
متن کاملMdivi-1 attenuates sodium azide-induced apoptosis in H9c2 cardiac muscle cells
The aim of the current study was to investigate the effect of mitochondrial division inhibitor 1 (Mdivi‑1) in sodium azide‑induced cell death in H9c2 cardiac muscle cells. Mdivi‑1 is a key inhibitor of the mitochondrial division protein dynamin‑related protein 1 (Drp1). Mdivi‑1 was added to H9c2 cells for 3 h, after which, the cells were treated with sodium azide for 24 h. Cell viability was me...
متن کاملarylaminotetrazoles : via [2+3] cycloaddition of nitriles and sodium azide via solid acid catalysis
The [3+2] cycloaddition between various nitriles and sodium azide proceeds smoothly in the presence of zeolite and sulfated Zircona as effective catalyst and in the water and DMF/MeOH, to give the corresponding arylaminotetrazoles in good to high yields. The reaction most probably precedes through the in situ formation of a catalyst azide species, followed by a successive [3+2] cycloaddition wi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Blood
دوره 61 4 شماره
صفحات -
تاریخ انتشار 1983